Difference between revisions of "SensNC 2018"

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== Method ==
 
== Method ==
  
Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined.
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Electrochemical Impedance Spectroscopy
  
 
==Molecular Recognition ==
 
==Molecular Recognition ==
  
The detection method is based on the absorbance properties that vancomycin possesses.  
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The vancomyocin is bound by its own reaction against D-ala-D-ala motif in a molecularly imprinted polymer, which is bound to the surface of the cartridge.
  
 
== Physical Transduction ==
 
== Physical Transduction ==
  
UV light (280 nm) was immited on a quartz cuvette. The light that is not absorbed passes through an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector.
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When the vancomyocin binds to the polymer, the characteristics of this polymer change, which changes the electrical impedance in a predictable way. This shifts the phase and amplitude of the alternating current that is put onto the cartridge
  
 
== Cartridge ==
 
== Cartridge ==
  
To plasma spiked with vancomycin, trichloroacetic acid (TCA) is added. The mixture is then centrifuged at 15000 rpm for 3 mins to separated proteins from plasma. Then, the supernatant is seperated from the precipitated proteins, and diluted to 1:10 volume ratio.
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The cartridge is disposable and the sample is pipetted onto it after mixing with the detection polyclonal antibodies.
  
 
== Reader Instrument ==
 
== Reader Instrument ==
  
No information is given
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The TMR sensor is linked to an arduino microcontroller, which calculates the concentration of vancomyocin and can link to an iOS app, update the patients records, connect to a printer to print out the records and display the measured concentration on an LCD screen.
 
 
== Prizes==
 
 
 
Public Inspiation Award
 

Latest revision as of 12:48, 21 August 2020

Logo of SenseNC


SensNC 2018

SensNC is a team competing in Sensus 2018, its university is the North Carolina State University. For Sensus 2018, SensNC investigated the possibilities for creating a biosensor which is able to measure the concentration of Vancomycin. The full TRD can be found via this link

Method

Electrochemical Impedance Spectroscopy

Molecular Recognition

The vancomyocin is bound by its own reaction against D-ala-D-ala motif in a molecularly imprinted polymer, which is bound to the surface of the cartridge.

Physical Transduction

When the vancomyocin binds to the polymer, the characteristics of this polymer change, which changes the electrical impedance in a predictable way. This shifts the phase and amplitude of the alternating current that is put onto the cartridge

Cartridge

The cartridge is disposable and the sample is pipetted onto it after mixing with the detection polyclonal antibodies.

Reader Instrument

The TMR sensor is linked to an arduino microcontroller, which calculates the concentration of vancomyocin and can link to an iOS app, update the patients records, connect to a printer to print out the records and display the measured concentration on an LCD screen.