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1. Knowledge: scientific and technical knowledge required to use the test. 2. Training and experience: training needed to perform the test. 3. Reagents and materials preparation: stability of materials and material preparation . 4. Characteristics of operational steps: complexity of operational steps. 5. Calibration, quality control and proficiently testing materials: can be stable or labile . 6. Test system troubleshooting and equipment maintenance: the easiness and frequency of the troubleshooting and equipment maintenance . 7. Interpretation and judgement: required to perform analytic processes and resolution of problems.
An alternative type of testing is with a ‘molecular assay’, which is more accurate than RITDs, because it detects viral RNA or nucleic acids in respiratory specimens. This type of testing includes rapid molecular assays, RT-PCR and other nucleic acid amplification tests. Molecular tests are mostly used in hospitals, some are able to detect both influenza A and B. Others can identify different subtypes. The ‘molecular assays’ are more accurate and sensitive than RIDTs, but the time-to-result may be several hours.<ref name="Arti28">Diagnosing Flu, CDC, 2020, https://www.cdc.gov/flu/symptoms/testing.htm</ref><ref name="Arti29">Information on Rapid Molecular Assays, RT-PCR, and other Molecular Assays for Diagnosis of Influenza Virus Infection, CDC, 2020, https://www.cdc.gov/flu/professionals/diagnosis/molecular-assays.htm</ref>
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==Safety==
When working with the influenza virus, influenza infection in humans can occur following a laboratory accident. For safety reasons, inactivated virus particles will be used in SensUs 2021, as a substitute for infectious influenza virus particles.
===Lab Protocols===
The use of safety equipment combined with good practices is fundamental to laboratory safety and in helping to reduce the risks involved in dealing with biosafety hazards.According to Article 4.84 of the Working Conditions Decree, the inactivated virus particles can be classified as category 1 of the biological agents<ref name="Arti30">1Short manual for theML-I, ML-II laboratory(gmo-labs) and/or laboratory for working with biological agents / human materials, University of Twente, 2018,https://www.utwente.nl/.uc/f4fcab199010209618400ac90d20284e61d13c337a78900/short-manual-gmo.pdf</ref>, as they are unlikely to cause disease in humans. Therefore, only the biosafety guidelines as defined in the ML-1 (minimum containment level) have to be followed. It is stipulated in the guidelines that general laboratory protocols such as the usage of barrier protection (lab coats, gloves and face protection) when handling the samples are to be followed. The protection barriers are not always necessary from a microbiological perspective. However, it is compulsory when handling disinfectants or solvents.
== References ==
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