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Traumatic Brain Injury

728 bytes added, 09:55, 22 December 2022
State of the Art
== State of the Art ==
Currently, the concentration of GFAP is typically measured in blood plasma (liquid component of blood in which blood cells are absent) or serum (plasma from which the clotting proteins have been removed). Classic methods for the detection of GFAP include ELISA (Enzyme-Linked Immunosorbent Assay).
 
{| class="wikitable" style="margin-bottom:0"
!Test Name
!Sample Volume (μL)
!Sample matrix
!Range (pg/mL)
!Precision (CV%)
!Incubation time (min)
!Measuring Technique
!Assay type
!Links
|-
|GFAP CLIA Assay, from Eagle Biosciences
|100
|serum, CSF
|10-640
|Intra-Assay: CV=1%, Inter-Assay: CV=6%
|150
|ELISA
|solid phase enzyme-linked sandwich immunosorbent
|https://eaglebio.com/product/gfap-clia-assay-kit/ https://eaglebio.com/wp-content/uploads/2022/01/gfp31-L01-gfap-clia-assay-kit-package-insert-2022.pdf
|-
|Roche COBAS
|5109442190
|Elecsys IL-6
|30
|Serum or plasma
|1.5 - 5000
|1.5
|N/A
|18
|ECLIA
|Sandwich
|-
|}<div style="margin-bottom:1em"><sub>''Table 1: Commercially available GFAP well-plate assays.</sub><br/>
== Lab protocols ==