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Adalimumab

31 bytes removed, 13:26, 10 May 2019
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To determine the trough levels of adalimumab, the samples must be taken within 24 hours prior to the drug administration.<ref name=”[27]”>MabTrack level adalimumab (2018). Leaflet ''MabTrack level adalimumab''. Sanquin.</ref>
These assays are sandwich-type assays with enzymatic labelling, expect for the RIDA®QUICK ADM Monitoring and Quantum Blue® Adalimumab, which are based on lateral flow immunoassays. Mabtrack by Sanquin uses polystyrene microtiter wells with immobilized TNF-specific mouse monoclonal antibodies. These bind recombinant TNF. The adalimumab that is present in the sample bind to the bound TNF on the microtiter plate and a adalimumab/TNF/anti-TNF complex is formed. Next, a monoclonal ADAb labeled with horseradish peroxidase is added which binds to the complex. A substrate solution leads to the formation of a colored product, proportional to the amount of adalimumab present in the sample.<ref name=”[27]”>MabTrack level adalimumab (2018). Leaflet ''MabTrack level adalimumab''. Sanquin.</ref> The RIDASCREEN ADM Monitoring and LISA-TRACKER Adalimumab both have immobilized TNF-⍺ on the surface of the microwell plate. While RIDASCREEN ADM the apDia makes use of ADAb conjugated with horseradish peroxidase to form a TNF-⍺/adalimumab/conjugated-ADAb complex, the LISA-TRACKER uses anti-human IgG biotinylated antibodies to form the complex, whereafter horseradish peroxydase labelled with streptavidin is added that binds to the complex.<ref name=”[28]”>RIDASCREEN ADM Monitoring apDia Adalimumab ELISA (2016). Leaflet ''RIDASCREEN ADM MonitoringapDia Adalimumab ELISA.'' R-Biopharm AGapDia.</ref><ref name=”[29]”>LTA002 LISA-TRACKER Adalimumab (2017). Leaflet ''LTA002 LISA-TRACKER Adalimumab''. Theradiag.</ref>
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