Difference between revisions of "Sandbox"

From SensUs Wiki
Jump to: navigation, search
Line 28: Line 28:
 
No information is given
 
No information is given
  
== Prizes if won ==
+
== Prizes==
  
 
Public Inspiation Award
 
Public Inspiation Award

Revision as of 21:28, 13 July 2020

Logo of Team Sanbox


Team Sandbox 2018

For Sensus 2018, Team Sandbox investigated the possibilities for creating a biosensor which is able to measure the concentration of Vancomycin. The full TRD can be found via this link

Method

Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined.

Molecular Recognition

The detection method is based on the absorbance properties that vancomycin possesses.

Physical Transduction

UV light (280 nm) was immited on a quartz cuvette. The light that is not absorbed passes through an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector.

Cartridge

To plasma spiked with vancomycin, trichloroacetic acid (TCA) is added. The mixture is then centrifuged at 15000 rpm for 3 mins to separated proteins from plasma. Then, the supernatant is seperated from the precipitated proteins, and diluted to 1:10 volume ratio.

Reader Instrument

No information is given

Prizes

Public Inspiation Award