[[File:sandbox.jpg|200px|thumb|right|Logo of Team Sanbox]]
== Sandbox ==
== Team Sandbox 2018 ==
This Team Sandbox is a sandboxteam competing in Sensus 2018, its university is the TU Eindhoven. For Sensus 2018, Team Sandbox investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/264/Team-Results-Document---AUC.pdf via this link] == Method == Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined. ==Molecular Recognition == The detection method is made based on the absorbance properties that vancomycin possesses. == Physical Transduction == UV light (280 nm) was immited on a quartz cuvette. The light that is not absorbed passes through an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector. == Cartridge == To plasma spiked with vancomycin, trichloroacetic acid (TCA) is added. The mixture is then centrifuged at 15000 rpm for 3 mins to separated proteins from plasma. Then, the supernatant is seperated from the precipitated proteins, and diluted to experiment1:10 volume ratio. == Reader Instrument == No information is given == Prizes== Public Inspiation Award
