Synosense is a team competing in Sensus 2019, its university is the KU Leuven. For Sensus 2019, Synosense investigated the possibilities for creating a biosensor which is able to measure the concentration of Adalimumab. The full TRD can be found via this link
Surface Plasmon Resonance
The gold (Au)-coated fiber optic probe is functionalized with capture antibodies against ADM using EDC/NHS chemistry on carboxylic acid self-assembling monolayers(SAM). When the functionalized fiber is brought in contact with the sample, ADM molecules bind to the capture antibodies.
Incident light is reflected inside the fiber through a phenomenon called total internal reflection (TIR). TIR occurs when light travels from a medium with higher refractive index (RI) (e.g.glass) to that with a lower RI(e.g.Au). The reflection at the medium interface creates surface plasmons: propagating electron density waves caused by interaction between incident photons and free electrons in the metal. The resonance caused by this wave delivers a decrease in the intensity of the reflected light at SPR wavelengths. This is referred to as the SPR dip. Because the SPR conditions depend on the RI in the vicinity of the interface, changes to the outer surface will result in shift of wavelengths required to meet the SPR condition. Thus, the consequent changes of the outer surface can be followed in real-time by detecting the shift in SPR wavelengths.
The Synochip is a disposable, robust, autonomous,inexpensive microfluidic chip, based on the SIMPLE technology, developed within the MeBioS-Biosensorsresearch group at KU Leuven.
The spectrometer and LED light are connected to and commanded by a Raspberry pi 3 model B which contains in-house written programs for the spectrometer, LED and screen control. The measurements are shown on a 7 inch Raspberry Touch display
Translational Potential Award