https://wiki.sensus.org/index.php?title=T.E.S.T._2019&feed=atom&action=historyT.E.S.T. 2019 - Revision history2024-03-29T15:30:25ZRevision history for this page on the wikiMediaWiki 1.29.2https://wiki.sensus.org/index.php?title=T.E.S.T._2019&diff=277&oldid=prevTechsensus at 13:02, 5 September 20202020-09-05T13:02:10Z<p></p>
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</table>Techsensushttps://wiki.sensus.org/index.php?title=T.E.S.T._2019&diff=275&oldid=prevTechsensus at 13:01, 5 September 20202020-09-05T13:01:00Z<p></p>
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</table>Techsensushttps://wiki.sensus.org/index.php?title=T.E.S.T._2019&diff=274&oldid=prevTechsensus at 12:59, 5 September 20202020-09-05T12:59:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Prizes ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Prizes ==</div></td></tr>
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</table>Techsensushttps://wiki.sensus.org/index.php?title=T.E.S.T._2019&diff=273&oldid=prevTechsensus at 12:59, 5 September 20202020-09-05T12:59:14Z<p></p>
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<td colspan='2' style="background-color: white; color:black; text-align: center;">Revision as of 12:59, 5 September 2020</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Physical Transduction ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Physical Transduction ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The chemical reaction occurs in a microfluidic cartridge. To convert the analogue light signal into a digital signal, the emitted light from a cartridge is led through an optical setup in a cage system. First, the light from the cartridge, which is considered as a point source, is collimated by a plano-convex lens (f=25.4 mm). The emission spectrum of the bioluminescent protein has two maxima as it consists of the signal from both mNeonGreen-Nanoluc and NanoBiT. For this reason, the spectrum is split at the minimum between these maxima into a green and a blue beam. This is done through means of a long-pass dichroic mirror at 490 nm at an angle of 45 degrees. Afterwards, the green and blue beams are focused (plano-convex, f=25.4 mm) on the photodiodes (Thorlabs PDF10A, Si fW Sensitivity Fixed Gain Detector 320-1100 nm). An overview of the optical setup is given in figure 2 on the next page. The photodiodes provide an analog signal that is converted with an ADC and processed in a Raspberry-Pi comput</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The chemical reaction occurs in a microfluidic cartridge. To convert the analogue light signal into a digital signal, the emitted light from a cartridge is led through an optical setup in a cage system. First, the light from the cartridge, which is considered as a point source, is collimated by a plano-convex lens (f=25.4 mm). The emission spectrum of the bioluminescent protein has two maxima as it consists of the signal from both mNeonGreen-Nanoluc and NanoBiT. For this reason, the spectrum is split at the minimum between these maxima into a green and a blue beam. This is done through means of a long-pass dichroic mirror at 490 nm at an angle of 45 degrees. Afterwards, the green and blue beams are focused (plano-convex, f=25.4 mm) on the photodiodes (Thorlabs PDF10A, Si fW Sensitivity Fixed Gain Detector 320-1100 nm). An overview of the optical setup is given in figure 2 on the next page. The photodiodes provide an analog signal that is converted with an ADC and processed in a Raspberry-Pi comput<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Cartridge ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Cartridge ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>For the sample handling a disposable cartridge with one chamber, an inlet and an outlet is used</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>For the sample handling a disposable cartridge with one chamber, an inlet and an outlet is used<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Reader Instrument ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Reader Instrument ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The used photodiodes provide an analog output signal between 0-10V.  A voltage divider is used to transform the actual voltage range to the range of 0-5V. This signal is converted by the ADC (ADS1115 16-bit) to a digital signal, so it can be processed by the Raspberry Pi (3B+). The photodiodes and Raspberry Pi are powered by one power supply (Farnell RPT-60B). A 7-inch LCD touchscreen makes it possible to interact with the device</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The used photodiodes provide an analog output signal between 0-10V.  A voltage divider is used to transform the actual voltage range to the range of 0-5V. This signal is converted by the ADC (ADS1115 16-bit) to a digital signal, so it can be processed by the Raspberry Pi (3B+). The photodiodes and Raspberry Pi are powered by one power supply (Farnell RPT-60B). A 7-inch LCD touchscreen makes it possible to interact with the device<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Prizes ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== Prizes ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Analytical Performance</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Analytical Performance <ins class="diffchange diffchange-inline">and </ins>Creativity Performance</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Creativity Performance</div></td><td colspan="2"> </td></tr>
</table>Techsensushttps://wiki.sensus.org/index.php?title=T.E.S.T._2019&diff=272&oldid=prevTechsensus: Created page with "Logo of Team Joint Venture == T.E.S.T 2019 == Team T.E.S.T. is a team competing in Sensus 2019, its university is the TU Eindhov..."2020-09-05T12:58:00Z<p>Created page with "<a href="/index.php?title=File:Jointventure.PNG" title="File:Jointventure.PNG">200px|thumb|right|Logo of Team Joint Venture</a> == T.E.S.T 2019 == Team T.E.S.T. is a team competing in Sensus 2019, its university is the TU Eindhov..."</p>
<p><b>New page</b></p><div>[[File:Jointventure.PNG|200px|thumb|right|Logo of Team Joint Venture]]<br />
<br />
<br />
== T.E.S.T 2019 ==<br />
<br />
Team T.E.S.T. is a team competing in Sensus 2019, its university is the TU Eindhoven. For Sensus 2019, T.E.S.T. investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Adalimumab]]. The full TRD can be found [https://digital2019.sensus.org/storage/523/T.E.S.T.-(The-Netherlands)---Team-Results-Document.pdf via this link]<br />
<br />
== Method ==<br />
<br />
Bioluminescence<br />
<br />
==Molecular Recognition ==<br />
<br />
The bioluminescent assay comprises two protein engineered and chemically modified components; tumor necrosis factor alpha (TNFα) and an anti-a dalimumab/TNFα (anti-AT) antibody (HCA204, Bio-Rad Laboratories). These components form the molecular recognition of the assay using the affinity of TNFα to adalimumab and subsequently anti-AT antibody to the adalimumab/TNFα complex (AT-complex).<br />
<br />
== Physical Transduction ==<br />
<br />
The chemical reaction occurs in a microfluidic cartridge. To convert the analogue light signal into a digital signal, the emitted light from a cartridge is led through an optical setup in a cage system. First, the light from the cartridge, which is considered as a point source, is collimated by a plano-convex lens (f=25.4 mm). The emission spectrum of the bioluminescent protein has two maxima as it consists of the signal from both mNeonGreen-Nanoluc and NanoBiT. For this reason, the spectrum is split at the minimum between these maxima into a green and a blue beam. This is done through means of a long-pass dichroic mirror at 490 nm at an angle of 45 degrees. Afterwards, the green and blue beams are focused (plano-convex, f=25.4 mm) on the photodiodes (Thorlabs PDF10A, Si fW Sensitivity Fixed Gain Detector 320-1100 nm). An overview of the optical setup is given in figure 2 on the next page. The photodiodes provide an analog signal that is converted with an ADC and processed in a Raspberry-Pi comput<br />
<br />
== Cartridge ==<br />
<br />
For the sample handling a disposable cartridge with one chamber, an inlet and an outlet is used<br />
<br />
== Reader Instrument ==<br />
<br />
The used photodiodes provide an analog output signal between 0-10V. A voltage divider is used to transform the actual voltage range to the range of 0-5V. This signal is converted by the ADC (ADS1115 16-bit) to a digital signal, so it can be processed by the Raspberry Pi (3B+). The photodiodes and Raspberry Pi are powered by one power supply (Farnell RPT-60B). A 7-inch LCD touchscreen makes it possible to interact with the device<br />
<br />
== Prizes ==<br />
<br />
Analytical Performance<br />
Creativity Performance</div>Techsensus