Difference between revisions of "UScience Tech Barcelona 2018"

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[[File:sandbox.jpg|200px|thumb|right|Logo of Team Sanbox]]
 
  
  
== Team Sandbox 2018 ==
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== UScience Tech Barcelona 2018 ==
  
  
UScience Tech Barcelona is a team competing in Sensus 2018, its university is the UVic and UPC. For Sensus 2018, UScience Tech Barcelona investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/265/Team-Results-Document---BarcelonaTech.pdf via this link]
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UScience Tech Barcelona is a team competing in Sensus 2018, its universities are the UVic and UPC. For Sensus 2018, UScience Tech Barcelona investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/265/Team-Results-Document---BarcelonaTech.docx via this link]
  
 
== Method ==
 
== Method ==
  
Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined.
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Impedance Spectroscopy
  
 
==Molecular Recognition ==
 
==Molecular Recognition ==
  
The detection method is based on the absorbance properties that vancomycin possesses.  
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The Vancomyocin is recognized by rabbit polyclonal antibodies that are added. This means that these antibodies can not bind to the surface of the detector that has also been coated with vancomyocin. (These rabbit antibodies are then recognized by anti-rabbit antibodies with Horse radish peroxidase attached.)
  
 
== Physical Transduction ==
 
== Physical Transduction ==
  
UV light (280 nm) was immited on a quartz cuvette. The light that is not absorbed passes through an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector.
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The concentration of vancomyocin is ultimately measured using impedance of two gold electrodes on the surface of the sensor when an alternating current is run through them. The unbound antibodies attach to these two electrodes, because they are coated with vancomyocin. These results can then be checked using colorimetry.
 
 
== Cartridge ==
 
 
 
To plasma spiked with vancomycin, trichloroacetic acid (TCA) is added. The mixture is then centrifuged at 15000 rpm for 3 mins to separated proteins from plasma. Then, the supernatant is seperated from the precipitated proteins, and diluted to 1:10 volume ratio.
 
 
 
== Reader Instrument ==
 
 
 
No information is given
 
 
 
== Prizes==
 
 
 
Public Inspiation Award
 

Latest revision as of 12:42, 26 August 2020


UScience Tech Barcelona 2018

UScience Tech Barcelona is a team competing in Sensus 2018, its universities are the UVic and UPC. For Sensus 2018, UScience Tech Barcelona investigated the possibilities for creating a biosensor which is able to measure the concentration of Vancomycin. The full TRD can be found via this link

Method

Impedance Spectroscopy

Molecular Recognition

The Vancomyocin is recognized by rabbit polyclonal antibodies that are added. This means that these antibodies can not bind to the surface of the detector that has also been coated with vancomyocin. (These rabbit antibodies are then recognized by anti-rabbit antibodies with Horse radish peroxidase attached.)

Physical Transduction

The concentration of vancomyocin is ultimately measured using impedance of two gold electrodes on the surface of the sensor when an alternating current is run through them. The unbound antibodies attach to these two electrodes, because they are coated with vancomyocin. These results can then be checked using colorimetry.