[[File:sandbox.jpg|200px|thumb|right|Logo of Team Sanbox]]
== Team Sandbox UScience Tech Barcelona 2018 ==
UScience Tech Barcelona is a team competing in Sensus 2018, its university is universities are the UVic and UPC. For Sensus 2018, UScience Tech Barcelona investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/265/Team-Results-Document---BarcelonaTech.pdf docx via this link]
== Method ==
Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined.Impedance Spectroscopy
==Molecular Recognition ==
The detection method Vancomyocin is based on recognized by rabbit polyclonal antibodies that are added. This means that these antibodies can not bind to the absorbance properties surface of the detector that vancomycin possesseshas also been coated with vancomyocin. (These rabbit antibodies are then recognized by anti-rabbit antibodies with Horse radish peroxidase attached. )
== Physical Transduction ==
UV light (280 nm) was immited on a quartz cuvette. The light that concentration of vancomyocin is not absorbed passes through ultimately measured using impedance of two gold electrodes on the surface of the sensor when an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector. == Cartridge == To plasma spiked with vancomycin, trichloroacetic acid (TCA) alternating current is addedrun through them. The mixture is then centrifuged at 15000 rpm for 3 mins unbound antibodies attach to separated proteins from plasmathese two electrodes, because they are coated with vancomyocin. Then, the supernatant is seperated from the precipitated proteins, and diluted to 1:10 volume ratioThese results can then be checked using colorimetry. == Reader Instrument == No information is given == Prizes== Public Inspiation Award
