Difference between revisions of "SensImperial 2018"

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[[File:Imperial.png|200px|thumb|right|Logo of Team Sanbox]]
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[[File:Imperial.png|200px|thumb|right|Logo of Imperial College of London]]
  
  
== Team Sandbox 2018 ==
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== SensImperial 2018 ==
  
  
Team Sandbox is a team competing in Sensus 2018, its university is the TU Eindhoven. For Sensus 2018, Team Sandbox investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/264/Team-Results-Document---AUC.pdf via this link]
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SensImperial is a team competing in Sensus 2018, its university is the Imperial College of London. For Sensus 2018, Team SensImperial investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Vancomycin]]. The full TRD can be found [https://digital.sensus.org/storage/258/Team-Results-Document---SensImperial.pdf via this link]
  
 
== Method ==
 
== Method ==
  
Spectrophotometry (Light absorbtion). Vanomycin has the property that it absorbs photons of a specific wavelength, the strength of the absorbtion is the dependant on the concentration. By looking at the transmission for a specific wavelenght of light the concentration of Vanomycin can be determined.
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Competitive Lateral Flow Assay
  
 
==Molecular Recognition ==
 
==Molecular Recognition ==
  
The detection method is based on the absorbance properties that vancomycin possesses.
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The detection method uses a large complex with a gold nanoparticle at the centre and vancomyocin and biotin on the outside, which have a bright red colour. When vancomyocin is present, this large complex is displaced from its original position on an vancomyocin antibody and binds to a line of streptavidin. This change in colour intensity signals the amount of vancomyocin in the serum
  
 
== Physical Transduction ==
 
== Physical Transduction ==
  
UV light (280 nm) was immited on a quartz cuvette. The light that is not absorbed passes through an optical filter (bandpass filter with center wavelength 300 nm) which then falls on a sicilone detector.
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The change in colour is detected by an app running a neural network, which is taught to detect the change in colour and convert it into a particular concentration of vancomyocin.  
  
 
== Cartridge ==
 
== Cartridge ==
  
To plasma spiked with vancomycin, trichloroacetic acid (TCA) is added. The mixture is then centrifuged at 15000 rpm for 3 mins to separated proteins from plasma. Then, the supernatant is seperated from the precipitated proteins, and diluted to 1:10 volume ratio.
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The plasma is pipetted onto the membrane, which then travels up the membrane, where it picks up the large complex and encounters the two lines of antibodies and streptavidin.
  
 
== Reader Instrument ==
 
== Reader Instrument ==
  
No information is given
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The reader is the mobile phone with the app, where the concentration is measured and saved
  
 
== Prizes==
 
== Prizes==
  
Public Inspiation Award
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None

Latest revision as of 21:29, 26 July 2020

Logo of Imperial College of London


SensImperial 2018

SensImperial is a team competing in Sensus 2018, its university is the Imperial College of London. For Sensus 2018, Team SensImperial investigated the possibilities for creating a biosensor which is able to measure the concentration of Vancomycin. The full TRD can be found via this link

Method

Competitive Lateral Flow Assay

Molecular Recognition

The detection method uses a large complex with a gold nanoparticle at the centre and vancomyocin and biotin on the outside, which have a bright red colour. When vancomyocin is present, this large complex is displaced from its original position on an vancomyocin antibody and binds to a line of streptavidin. This change in colour intensity signals the amount of vancomyocin in the serum

Physical Transduction

The change in colour is detected by an app running a neural network, which is taught to detect the change in colour and convert it into a particular concentration of vancomyocin.

Cartridge

The plasma is pipetted onto the membrane, which then travels up the membrane, where it picks up the large complex and encounters the two lines of antibodies and streptavidin.

Reader Instrument

The reader is the mobile phone with the app, where the concentration is measured and saved

Prizes

None