Difference between revisions of "SensSwiss 2018"

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Revision as of 11:54, 28 July 2020

Logo of Team Sanbox


SensSwiss 2018

Direct Fluorescence Polarization

Method

Direct Fluorescence Polarization

Molecular Recognition

The fluorescently-labeled probe molecule is a peptide coupled to a fluorophore. A peptide precursor of the bacterial cell wall is used, L-Lys-D-Ala-D-Ala (KAA) moiety of Lipid II, coupled to a fluorophore: fluorescein isothuicyanate (FITC).

Physical Transduction

The fluorescent molecule is excited with plane-polarized light, causing the molecule to emit polarized light in the same plane of polarization. If the fluorescent probe is bound to Vancomycin, the complex is able to retain the polarization of light for longer than an unbound probe. The signal is detected through a polarizing filter. The degree of polarization detected is linked to the amount of target molecule in the sample.

Cartridge

Cartridge consists of 3 wells, made of PMMA, transparent and non autofluorescent. Those wells are heated and scanned.

Reader Instrument

The user interface of our device consists of a touch screen, communicating with the Arduino to coordinate different tasks, such as the heating and scanning steps required for the completion of an accurate vancomycin concentration measurement. ...

Prizes

Analytical Performance