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== BiosensUM 2019 ==
BiosensUM is a team competing in Sensus 2019, its universities are Université de Montréala–HEC Montréalb and Polytechnique Montréal. For Sensus 2019, BiosensUM investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Adalimumab]]. The full TRD can be found [https://digital2019.sensus.org/storage/515/BiosensUM-(Canada)---Team-Results-Document.pdf via this link]
== Method ==
Surface Plasmon Resonance
==Molecular Recognition ==
In order to specifically detect adalimumab, we functionalized the gold-coated prism according to an established protocol optimized for antibody sensing in plasma with a self-assembly monolayer (SAM)5to which we attached TNF alpha, the highly specific ligand of adalimumab. The molecule used for the SAM is a short hexa-peptide with a thiol functional group at the N-terminal extremity (3-MPA-LHDLHD-OH) (1mg/mL in DMF) that will covalently bond to the gold layerand will form a dense peptide layer after 16h of incubation
== Physical Transduction ==
The refractive index increase caused by the binding of adalimumab with TNF-alpha at the gold-layer surface causes a shift in the λ. A typical SPR instrument would be equipped with a spectrophotometer that can accurately measure wavelength shifts. However, such a detector is bulky, expensive and not user-friendly since it requires to be connected to a computer to process the data. We developed an SPR system using a cell phone LED light as the incident light source and the CCD camera as a detector by taking a simple picture. The CCD camera will not measure a wavelength shift;however, we can detect a corresponding pixel shift in the captured image with the custom algorithm integrated in our phone application.
==Cartridge==
We developed a microfluidic chip maximizing the area ofdetection while minimizing the volume of sample. Our cartridge is 2cm by 2cm by 1cmand made in polycarbonateand is sealed to the gold-coated prism. The sample is injected into the input channel and suction is then applied at the output channel using a small syringe to induce a controlled flow. This system allows multiple injections and washing steps required for the surface functionalization. Another important feature is the tight seal that forms between the microfluidic chip,the gold-coated prismand the syringe, which reduces the risk of leaks and contamination.
== BiosensUM 2019 ==
BiosensUM is a team competing in Sensus 2019, its universities are Université de Montréala–HEC Montréalb and Polytechnique Montréal. For Sensus 2019, BiosensUM investigated the possibilities for creating a biosensor which is able to measure the concentration of [[Adalimumab]]. The full TRD can be found [https://digital2019.sensus.org/storage/515/BiosensUM-(Canada)---Team-Results-Document.pdf via this link]
== Method ==
Surface Plasmon Resonance
==Molecular Recognition ==
In order to specifically detect adalimumab, we functionalized the gold-coated prism according to an established protocol optimized for antibody sensing in plasma with a self-assembly monolayer (SAM)5to which we attached TNF alpha, the highly specific ligand of adalimumab. The molecule used for the SAM is a short hexa-peptide with a thiol functional group at the N-terminal extremity (3-MPA-LHDLHD-OH) (1mg/mL in DMF) that will covalently bond to the gold layerand will form a dense peptide layer after 16h of incubation
== Physical Transduction ==
The refractive index increase caused by the binding of adalimumab with TNF-alpha at the gold-layer surface causes a shift in the λ. A typical SPR instrument would be equipped with a spectrophotometer that can accurately measure wavelength shifts. However, such a detector is bulky, expensive and not user-friendly since it requires to be connected to a computer to process the data. We developed an SPR system using a cell phone LED light as the incident light source and the CCD camera as a detector by taking a simple picture. The CCD camera will not measure a wavelength shift;however, we can detect a corresponding pixel shift in the captured image with the custom algorithm integrated in our phone application.
==Cartridge==
We developed a microfluidic chip maximizing the area ofdetection while minimizing the volume of sample. Our cartridge is 2cm by 2cm by 1cmand made in polycarbonateand is sealed to the gold-coated prism. The sample is injected into the input channel and suction is then applied at the output channel using a small syringe to induce a controlled flow. This system allows multiple injections and washing steps required for the surface functionalization. Another important feature is the tight seal that forms between the microfluidic chip,the gold-coated prismand the syringe, which reduces the risk of leaks and contamination.
