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|}<div style="margin-bottom:1em"><sub>''Table 1: Commercially available IL-6 well-plate assays.<br />
Immunoassays, in general, provide a relatively slow method for measuring specific biomarkers like IL-6. Therefore, faster methods are the subject of intensive research. A commercially available rapid test is the Milenia® QuickLine IL-6 test: Milenia Biotec.<ref name="Arti27">Milenia Biotec, 2016, https://www.milenia-biotec.com/en/product/il6/#nav-overview </ref> This lateral flow immunoassay is designed for the semi-quantitative evaluation of human Interleukin-6 in serum, plasma, cell culture supernatant, amniotic fluid or cerebrospinal fluid.<ref name="Arti28">Rapid and sensitive detection of interleukin-6 in serum via time-resolved lateral flow immunoassay, Analytical Biochemistry, 588, 113468, 2020, Huang, D., Ying, H., Jiang, D., Liu, F., Tian, Y., Du, C., Zhang, L., & Pu, X., https://doi.org/10.1016/j.ab.2019.113468</ref><ref name="Arti29">Development of quantum dot-based fluorescence lateral flow immunoassay strip for rapid and quantitative detection of serum interleukin-6, Journal of Clinical Laboratory Analysis, 35(5), e23752, 2021, Tang, J., Wu, L., Lin, J., Zhang, E., & Luo, Y., https://doi.org/10.1002/jcla.23752 </ref> In the Milenia® QuickLine IL-6 test, the sample is pipetted in the sample application port. IL-6 of the patient's sample binds to a first monoclonal anti-IL-6 antibody conjugated to gold particles. The IL-6-loaded gold particles diffuse through the membrane and overflow the test line (T). There, a second monoclonal antibody specific for IL-6 is coated on the membrane; so the gold particles are bound specifically and become visible as a colored line. The color intensity is proportional to the concentration of IL-6 in the sample and intensifies during the incubation time. The surplus of gold particles continues to diffuse over the test strip. The conjugate specific antibodies printed as a control line on the membrane (control line, C) capture the gold conjugate and therefore a visible line develops during the incubation time.<ref name="Arti30">A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics, J Clin Med Res, 2(2), 1–16, 2020, de Souza Sene, I., Costa, V., Bras, D. C., de Oliveira Farias, E. A., Nunes, G. E., & Bechtold, I. H., https://doi.org/https://doi.org/10.37191/Mapsci-2582-4333-2(2)-032 </ref><ref name="Arti31">Duplex Shiny app quantification of the sepsis biomarkers C-reactive protein and interleukin-6 in a fast quantum dot labeled lateral flow assay, Journal of Nanobiotechnology, 18(1), 130, 2020, Ruppert, C., Kaiser, L., Jacob, L. J., Laufer, S., Kohl, M., & Deigner, H.-P., https://doi.org/10.1186/s12951-020-00688-1 </ref>
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